Protease Inhibitor Cocktail (100X) from Cell Signaling Technology

Product Specs
ItemProtease Inhibitor Cocktail (100X)
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number5871S
Quantity1 ml
Research AreaRelated Products
Clone100X
ApplicationsW

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) A Novel Form of Neuregulin 1 Type III Caused by N-Terminal ProcessingBiomoleculesDecember 7, 2023Yukai Wang, Yu Zhang, Yingxing Wang, Hong Chen, Liangjing Pan, Xufeng Liao, Shunqi Wangsupplemented with 1% PMSF (Phenyl-methane sulfonyl fluoride) and protease inhibitor cocktail (Cat. #5871, Cell Signaling Technology, Danvers, MA, USA) before use. After electrophoresis, samples were transferred

(2) An optimized protocol to detect ubiquitination modification of exogenous or endogenous proteinsSTAR ProtocolsDecember 1, 2023Xinyu Li, Wei Jiang, Min FangProtein G PLUS-Agarose,Santa Cruz,Cat# sc-2002 Protease inhibitor cocktail,Cell Signaling Technology,Cat# 5871S PMSF,Lablead,Cat# P0754 Cell lysis buffer for western and IP (WB-IP),Beyotime,Cat# P0013 MG132,Sigma,Cat#

(3) Celecoxib/Cyclodextrin Eye Drop Microsuspensions: Evaluation of In Vitro Cytotoxicity and Anti-VEGF Efficacy for Retinal DiseasesPharmaceuticsNovember 28, 2023Phatsawee Jansook, Hay Man Saung Hnin Soe, Rathapon Asasutjarit, Theingi Tun, Hay Marn Hnin, Phyo Darli Maw, Tanapong Watchararot, Thorsteinn LoftssonCell Signaling Technology, Danvers, MA, USA), supplemented with a protease inhibitor cocktail (100×, cat. no. 5871; Cell Signaling Technology, Danvers, MA, USA). The lysed cells were then centrifuged at 4 °C, 12,000

(4) The thiol methyltransferase activity of TMT1A (METTL7A) is conserved across speciesbioRxivNovember 22, 2023José M. González Dalmasy, Christina M. Fitzsimmons, William J.E. Frye, Andrew J. Perciaccante, Connor P. Jewell, Lisa M. Jenkins, Pedro J. Batista, Robert W. Robey, Michael M. Gottesman0.1% SDS, 2 mM EDTA, and 0.5% deoxycholate, 50 mM NaCl) containing protease inhibitor cocktail (cat# 5871, Cell Signaling Technology, Danvers, MA). To inhibit residual histone deacetylase (HDAC) activity after

(5) Xinyang tablet ameliorates sepsis-induced myocardial dysfunction by regulating Beclin-1 to mediate macrophage autophagy and M2 polarization through LncSICRNT1 targeting E3 ubiquitin ligase TRAF6Chinese MedicineNovember 2, 2023Yuanyuan Luo, Yuanmei Li, Liwei He, Haitao Tu, Xinfeng Lin, Fengli Zhao, Yusheng Huang, Minyong Wen, Lingjun Wang, Zhongqi Yanglysis buffer (#89,900, Thermo Fisher Scientific) supplemented with a protease inhibitor cocktail (#5871, Cell Signaling Technology, Shanghai, China). The quantification of extracted proteins was conducted

(6) A gut commensal bacterium promotes black soldier fly larval growth and development partly via modulation of intestinal protein metabolismmBioOctober 31, 2023Xingyu Luo, Gangqi Fang, Kuangqin Chen, Yu Song, Tianyi Lu, Jeffery K. Tomberlin, Shuai Zhan, Yongping Huangpurchased from Beyotime (Catalog #: SG2000). PIC was purchased from Cell Signaling Technology (Product #: 5871). Wheat bran was purchased from a local farmer market. Bacterial isolation and bacterial association

(7) Heterozygous nonsense variants in the ferritin heavy-chain gene FTH1 cause a neuroferritinopathyHuman Genetics and Genomics AdvancesOctober 1, 2023Joseph T. Shieh, Jesus A. Tintos-Hernandez, Chaya N. Murali, Monica Penon-Portmann, Marco Flores-Mendez, Adrian Santana, Joshua A. Bulos, Kang Du, Lucie Dupuis, Nadirah Damseh, et al.detergent, 120 mM NaCl, 40 mM HEPES pH 7.5, 50 mM NaF, 2 mM NaVO3, protease inhibitor cocktail [CST 5871]). Protein concentration was measured with the Bradford reagent (Bio-Rad). Cell lysates (30 μg) were

(8) Selective Activation of M1 Muscarinic Receptors Attenuates Human Colon Cancer Cell ProliferationCancers (Basel)September 28, 2023Margaret H. Sundel, Natalia Sampaio Moura, Kunrong Cheng, Oscar Chatain, Shien Hu, Cinthia B. Drachenberg, Guofeng Xie, Jean-Pierre Raufmancells were lysed in a solution containing Cell Lysis Buffer (catalog #9803), anti-protease (catalog #5871), and anti-phosphatase (catalog #5870)—all from Cell Signaling. Cell lysates were centrifuged at 15,000×

(9) NRP1 transduces mechanical stress inhibition via LATS1/YAP in hypertrophic scarsCell Death DiscoverySeptember 13, 2023Mengzhi Li, Peng Wang, Jingting Li, Fei Zhou, Shixin Huang, Shaohai Qi, Bin ShuWaltham, MA, USA) containing a protease inhibitor cocktail and phosphatase inhibitor cocktail (1:100; #5871 and #5870, respectively; Cell Signaling Technology, Danvers, MA, USA). Briefly, 20 µl of each sample

(10) 1,25(OH)(2)D(3) ameliorates doxorubicin‑induced cardiomyopathy by inhibiting the NLRP3 inflammasome and oxidative stressExperimental and therapeutic medicineSeptember 1, 2023Gu X, Zhao L, Ye J, Chen L, Sui C, Li B, Wang X, Zhang J, Du Y.mM HEPES (pH=7.4), 10 mM EDTA, 0.5 mM EGTA, 0.1% SDS and 1X Protease Inhibitor Cocktail (cat. no. #5871; Cell Signaling Technology). After incubating for 10 min on ice, the cells were transferred into a 15-ml

(11) Tau seeding and spreading in vivo is supported by both AD-derived fibrillar and oligomeric tauActa NeuropathologicaAugust 1, 2023Anastasie Mate De Gerando, Lindsay A. Welikovitch, Anita Khasnavis, Caitlin Commins, Calina Glynn, Joshua E. Chun, Romain Perbet, Bradley T. HymanDounce homogenized in 5 v/w buffer containing phosphate buffer saline (PBS) and 1X protease inhibitor (#5871, Cell signaling) in a 15 ml glass homogenizer with 30 up and down strokes at 70% power by hand on ice.

(12) Terfezia boudieri and Terfezia claveryi inhibit the LPS/IFN-γ-mediated inflammation in RAW 264.7 macrophages through an Nrf2-independent mechanismScientific ReportsJune 21, 2023Abdelhameed S. Dawood, Mohamed S. Sedeek, Mohamed A. Farag, Anwar AbdelnaserJunction, NJ, USA). Cell Lysis Buffer (10X; Cat. No. 9803S), Protease Inhibitor Cocktail (100X; Cat. No. 5871), and Prestained Protein Marker, Broad Range (11–190 kDa; Cat. No. 13953S) were purchased from Cell Signaling

(13) Impact of HepG2 Cells Glutathione Depletion on Neutral Sphingomyelinases mRNA Levels and ActivityCurrent Issues in Molecular BiologyJune 8, 2023Marie Gamal, Hatem Tallima, Hassan M. E. Azzazy, Anwar Abdelnaserwas purchased from Oxford Lab Fine Chem LLP (Maharashtra, India). Protease inhibitor cocktail (100×) (5871) was purchased from Cell Signaling Technology (Danvers, MA, USA). 2.2. Cell Cultures HepG2 cells were

(14) YB1 modulates the DNA damage response in medulloblastomaScientific reportsMay 19, 2023McSwain LF, Pillsbury CE, Haji-Seyed-Javadi R, Rath SK, Chen V, Huang T, Shahab SW, Kunhiraman H, Ross J, Price GA, et al.Western blotting Tissues or cells were homogenized and lysed in RIPA lysis buffer with protease inhibitor cocktail (CST 5871), and phosphatase inhibitors. A total of 15–30 μg of each sample was denatured and separated on 14%

(15) Nono deficiency impedes the proliferation and adhesion of H9c2 cardiomyocytes through Pi3k/Akt signaling pathwayScientific ReportsMay 2, 2023Yu-Qing Lei, Zhou-Jie Ye, Ya-Lan Wei, Li-Ping Zhu, Xu-Dong Zhuang, Xin-Rui Wang, Hua Caono. 89900), which encompassed the Protease Inhibitor Cocktail (Cell Signaling Technology, cat. no. 5871). Halt™ Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific, cat. no. 78420) was used to preserve

(16) PROTAC-Mediated Selective Degradation of Cytosolic Soluble Epoxide Hydrolase Enhances ER Stress ReductionACS Chemical BiologyApril 21, 2023Yuxin Wang, Christophe Morisseau, Akihiro Takamura, Debin Wan, Dongyang Li, Simone Sidoli, Jun Yang, Dennis W. Wolan, Bruce D. Hammock, Seiya Kitamuracold phosphate-buffered saline (PBS) and lysed using RIPA buffer with protease inhibitor cocktail (#5871S, Cell Signaling, Danvers, MA) and phosphatase inhibitor cocktail (#78420, Life Technologies, Carlsbad,

(17) CD73-Dependent Adenosine Signaling through Adora2b Drives Immunosuppression in Ductal Pancreatic CancerCancer ResearchApril 4, 2023Erika Y. Faraoni, Kanchan Singh, Vidhi Chandra, Olivereen Le Roux, Yulin Dai, Ismet Sahin, Baylee J. O'Brien, Lincoln N. Strickland, Le Li, Emily Vucic, et al.(Cell Signaling Technology; #9803S) with protease inhibitor cocktail tablets (Cell Signaling Technology; #5871). The BCA method (Thermo Fisher Scientific) was used for protein quantification. Protein (20 μg) was

(18) The CTLA‐4 immune checkpoint protein regulates PD‐L1:PD‐1 interaction via transendocytosis of its ligand CD80The EMBO JournalMarch 1, 2023Alan Kennedy, Maximillian A Robinson, Claudia Hinze, Erin Waters, Cayman Williams, Neil Halliday, Simon Dovedi, David M SansommM NaCl, 10 mM EDTA and 1% Triton x‐100 supplemented with Protease Inhibitor Cocktail (cat. Number: 5871, Cell Signalling Technology). After 30 min incubation at 4°C with gentle rotation, lysates were spun

(19) Heterozygous Nonsense Variants in the Ferritin Heavy Chain Gene FTH1 Cause a Novel Pediatric NeuroferritinopathymedRxivFebruary 3, 2023Joseph T Shieh, Jesus A Tintos-Hernández, Chaya N. Murali, Monica Penon-Portmann, Marco Flores-Mendez, Adrian Santana, Joshua A. Bulos, Kang Du, Lucie Dupuis, Nadirah Damseh, et al.(30% CHAPS, 120 mM NaCl, 40 mM HEPES pH 7.5, 50 mM NaF, 2 mM NaVO3, protease inhibitor cocktail [CST 5871]). Protein concentration was measured with the Bradford reagent (Bio-Rad). Cell lysates (30 μg) were

(20) LncRNA SNHG6 Upregulates KPNA5 to Overcome Gemcitabine Resistance in Pancreatic Cancer via Sponging miR-944Pharmaceuticals (Basel)January 25, 2023Ge Gao, Xin Li, Hui Wu, Ling-li Huang, Yu-xin Lin, Zhi Huo, Zhong-yuan Xiang, Xiao ZhouThermo Fisher, Waltham, MA, USA). Protein was extracted by RIPA buffer supplemented with proteinase inhibitor (5871, Cell Signaling Technology, Danvers, MA, USA). This study was approved by the Human Ethics Review Committee

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Protease Inhibitor Cocktail (100X) from Cell Signaling Technology

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Protease Inhibitor Cocktail (100X) from Cell Signaling Technology

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